Isolation of microorganisms and lipase production study using agricultural residues

Authors

  • Mayana Barreto da Silva Universidade Federal de Sergipe
  • Diego Fonseca Bispo Universidade Federal de Sergipe
  • Rogério Luz Pagano Universidade Federal de Sergipe
  • Antonio Santos Silva Universidade Federal de Sergipe
  • Edilson Jesus Santos Universidade Federal de Sergipe
  • Cristina Ferraz Silva Universidade Federal de Sergipe Centro de Ciências Exatas e Tecnologia Departamento de Engenharia Química Laboratório de Bioquímica Industrial

DOI:

https://doi.org/10.14808/sci.plena.2016.054214

Keywords:

Lipase, Microorganism, Isolation

Abstract

Lipases are enzymes that have been highlighted in the biotechnology scenario, mainly due to their ability to catalyze hydrolysis and synthesis reactions. Lipases are produced by microorganisms, animals and plants, but the microbial origin is currently the most widely used industrially. Among the lipase producers microorganisms are filamentous fungi, bacteria and yeasts. This study aimed to isolation and selection of bacteria producing lipase and the enzyme production study using agro industrial wastes. Samples for isolation were collected in a biodiesel pilot plant. Microorganisms were isolated and then subjected to testing for lipase activity. After the selection process, the isolates showed greater enzymatic index (EI) were used for the study of lipase production using bagasse from sugarcane and cassava bagasse. In this studied were isolated 16 microorganisms, where 75% presented lipolytic activity. Among the isolates selected as lipase producers, the maximum production was achieved with the 8A strain when used bagasse cassava for 48 h fermentation.

Author Biographies

Mayana Barreto da Silva, Universidade Federal de Sergipe

Departamento de Engenharia Química
Laboratório de Bioquímica Industrial

Diego Fonseca Bispo, Universidade Federal de Sergipe

Departamento de Engenharia Química
Laboratório de Bioquímica Industrial

Rogério Luz Pagano, Universidade Federal de Sergipe

Departamento de Engenharia Química

Antonio Santos Silva, Universidade Federal de Sergipe

Departamento de Matemática

 

Edilson Jesus Santos, Universidade Federal de Sergipe

Departamento de Engenharia Química

Cristina Ferraz Silva, Universidade Federal de Sergipe Centro de Ciências Exatas e Tecnologia Departamento de Engenharia Química Laboratório de Bioquímica Industrial

Departamento de Engenharia Química
Laboratório de Bioquímica Industrial

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Published

2016-05-12

How to Cite

da Silva, M. B., Bispo, D. F., Pagano, R. L., Silva, A. S., Santos, E. J., & Silva, C. F. (2016). Isolation of microorganisms and lipase production study using agricultural residues. Scientia Plena, 12(5). https://doi.org/10.14808/sci.plena.2016.054214

Issue

Vol. 12 No. 5 (2016): May/Maio 2016

Section

VII Seminário de Pesquisa em Engenharia Química - Edição financiada pela CAPES

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