In vitro selection of isolates from the gastrointestinal tract of tambaqui, Colossoma macropomum, with probiotics potential
DOI:
https://doi.org/10.14808/sci.plena.2023.056201Keywords:
gut bacteria, antagonism, autochthonousAbstract
Probiotics have been used for more than 30 years in aquaculture, however, better results have been demonstrated using species-specific strains, supporting the premise of not using allochthonous probiotics. For this reason, the objective of this study was to evaluate the probiotic potential of strains of lactic acid bacteria from the intestinal microbiota of tambaqui. The isolated bacteria were identified according to Vieira et al. (2013), through the Gram reaction, morphology, gas production, catalase and hemolysis, being tested for their resistance to pH, the presence of bile salts and NaCl, in addition to antagonism against pathogens and resistance profile. 28 bacterial strains were isolated, of which only four (CP03, CP08, CP10 and CP20) were selected in the initial screening based on the characteristics of lactic acid bacteria. Strains CP20 and CP03 were the ones that best responded to tolerance tests for bile salts, pH and presence of NaCl respectively. CP03 showed antagonism only against Staphylococcus aureus and CP08 and CP20 showed antagonism against Escherichia coli, Vibrio harveyi, Pseudomonas aeroginosa and Aeromonas hydrophila. Only CP03 and CP020 were sensitive to all tested antibiotics. Bacterial strains CP03 and CP020 exhibited significant probiotic properties, showing good viability to in vitro resistance tests, as well as pathogen inhibitory capacity. Thus being recommended as a possible multispecies probiotic.
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Copyright (c) 2023 Antonio Carlos Freitas Souza, Jaqueline Freitas Souza, Manoel Daltro Nunes Garcia Júnior, Tiago Cunha Rocha, Jorge Belém Oliveira Júnior, Priscila Soares Sabbadini, Francisca Neide Costa
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