Enzymatic and antimicrobial activity of microorganisms isolated from a cave in the Amazon region

Authors

DOI:

https://doi.org/10.14808/sci.plena.2023.046201

Keywords:

Carajás, iron lithology, cave microorganisms

Abstract

Cave microorganisms, mainly in iron lithology, are poorly known. These constitute most of the cave's biodiversity and play a key role in maintaining the cave ecosystem. The isolation and characterization of the cave microbiota can lead to identifying new species and obtaining substances of biotechnological interest. This work aimed to isolate and evaluate the enzymatic and antimicrobial activity of microorganisms isolated from the cave soil of Parque Nacional dos Campos Ferruginosos National Park, southeastern Amazon. Soil samples were collected from the inner surface layer of cave GEM-1462 in different zones (aphotic, dysphotic and photic). To characterize the strains, the Gram stain technique and biochemical test were used. The microorganisms' proteolytic, cellulolytic, amylolytic, nitrogen fixation, phosphate solubilization, and actinomycete activities were evaluated. Antimicrobial activity was evaluated by antibiosis assay, disk diffusion test and minimal inhibitory concentration against standard bacterial strains. In total, 10 distinct microbial colonies were isolated and all showed enzymatic activity, highlighting the proteolytic activity. Four microbial strains showed antimicrobial activity against Bacillus subtilis and Klebsiella pneumoniae strains. This study demonstrated the first discovery of biological activity of iron cave microorganisms, indicating that the GEM-1462 cave is a promising source for prospecting microorganisms with biotechnological potential, mainly of proteolytic enzymes.

Author Biographies

Brenda Almeida Lima, Universidade Estadual do Oeste do Paraná

Mestranda do Programa de Pós-Graduação em Conservação e Manejo de Recursos Naturais, Universidade Estadual do Oeste do Paraná.

Rafael dos Santos Scherer, Grupo Espeleológico de Marabá

Membro do Grupo Espeleológico de Marabá, Pará.

Ulisses Brigatto Albino, Universidade Federal do Sul e Sudeste do Pará

Docente do Programa de Pós-Graduação em Química, Universidade Federal do Sul e Sudeste do Pará (Unifesspa).

Felipe Fernando da Silva Siqueira, Universidade Federal do Sul e Sudeste do Pará

Docente do curso de Biologia da Universidade Federal do Sul e Sudeste do Pará (Unifesspa).

José Augusto Pires Bitencourt, Instituto Tecnológico Vale

Pesquisador do Instituto Tecnológico Vale (ITV), Belém.

Sidnei Cerqueira dos Santos, Universidade Federal do Sul e Sudeste do Pará

Doutor em Biotecnologia. Professor Adjunto do curso de Ciências Biológicas do Instituto de Estudos em Saúde e Biológicas da Universidade Federal do Sul e Sudeste do Pará. Atua na área de Biotecnologia, Microbiologia e Propriedade Intelectual. Endereço para acessar este CV: http://lattes.cnpq.br/5913484433989475

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Published

2023-05-16

How to Cite

Almeida Lima, B., dos Santos Scherer, R. ., Brigatto Albino, U. ., da Silva Siqueira, F. F. ., Pires Bitencourt, J. A. ., & Cerqueira dos Santos, S. . (2023). Enzymatic and antimicrobial activity of microorganisms isolated from a cave in the Amazon region . Scientia Plena, 19(4). https://doi.org/10.14808/sci.plena.2023.046201

Issue

Vol. 19 No. 4 (2023): Abril/April 2023

Section

Articles

License

Copyright (c) 2023 Brenda Almeida Lima, Rafael dos Santos Scherer, Ulisses Brigatto Albino, Felipe Fernando da Silva Siqueira, José Augusto Pires Bitencourt, Sidnei Cerqueira dos Santos

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