Enzyme-assisted extraction of phenolic compounds from murucizeiro leaves (Byrsonima crassifolia)

Johnatt Allan Rocha Oliveira; Andrea Komesu; Luiza Helena Da Silva Martins; Hervé Rogez; Rosinelson da Silva Pena

doi:10.14808/sci.plena.2020.051501

Enzyme-assisted extraction of phenolic compounds from murucizeiro leaves (Byrsonima crassifolia)

Authors

Johnatt Allan Rocha Oliveira Universidade Federal do Pará https://orcid.org/0000-0002-1778-3043
Andrea Komesu Universidade Estadual de Campinas https://orcid.org/0000-0002-2961-5637
Luiza Helena Da Silva Martins Universidade Federal do Pará https://orcid.org/0000-0003-1911-4502
Hervé Rogez Universidade Federal do Pará https://orcid.org/0000-0001-8925-8881
Rosinelson da Silva Pena Universidade Federal do Pará https://orcid.org/0000-0002-3900-205X

DOI:

https://doi.org/10.14808/sci.plena.2020.051501

Keywords:

Byrsonima crassifolia, phenolic compounds, Response surface methodology

Abstract

The aim of this work was to evaluate the enzyme-assisted extraction of phenolic compounds from murucizeiro leaves (Byrsonima crassifolia) employing response surface methodology (RSM), in order to determine the best extraction conditions. The chemical composition of the leaves and characteristics of the enzymes were determined. The independent variables were temperature (19 to 70°C), pH (2.4 to 7.5) and reaction time (19 to 220 minutes). A conventional and optimized triple extraction with organic solvents was used as control. As result to solvent extraction was observed 73.28 mg of gallic acid equivalents (GAE) g^-1 DM, while under the best condition for enzyme-assisted extraction (temperature: 60°C; pH: 4.5; and time: 220 min), the concentration was 90.65 mg GAE g^-1. The independent variables significant were: temperature, pH and log time at the linear level and temperature at the quadratic level. A similar methodology could be encouraged for other natural products rich in phenolic compounds.

Author Biographies

Johnatt Allan Rocha Oliveira, Universidade Federal do Pará

Faculdade de Nutrição, Instituto de Ciências da Saúde, Universidade Federal do Pará

Andrea Komesu, Universidade Estadual de Campinas

Faculdade de Engenharia Química

Luiza Helena Da Silva Martins, Universidade Federal do Pará

Faculdade de Engenharia de Alimentos

Hervé Rogez, Universidade Federal do Pará

Instituto de Ciências Biológicas, Faculdade de Biotecnologia

Rosinelson da Silva Pena, Universidade Federal do Pará

Faculdade de Engenharia de Alimentos, Instituto de Tecnologia

Downloads

Published

2020-06-23

How to Cite

Oliveira, J. A. R., Komesu, A., Martins, L. H. D. S., Rogez, H., & Pena, R. da S. (2020). Enzyme-assisted extraction of phenolic compounds from murucizeiro leaves (Byrsonima crassifolia). Scientia Plena, 16(5). https://doi.org/10.14808/sci.plena.2020.051501

Download Citation

Issue

Vol. 16 No. 5 (2020): Maio/May 2020

Section

Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work