Addition of freeze-dried pulp of Noni in diluent for semen freezing on the integrity of the plasma membrane of sperm ram

Abstract

This study aimed to verify the effect of the addition of freeze-dried pulp concentrations of Noni fruit (Morinda citrifolia Linn) in semen diluent, on the integrity of the plasma membrane of sperm sheep, evaluated by hiposmotic test (HOST) in five ejaculates rams Santa Inês (SI). The treatments consisted of D1 (control without addition of freeze-dried pulp) D2, D3 and D4 with the addition of freeze-dried pulp (2,35mg / mL; 4,70mg / mL and 7,05mg / mL respectively). The samples were frozen, thawed (37 ° C for 30 sec), incubated in hyposmotic solution and subjected to analysis in the integrity of the plasma membrane by the HOST and TTR immediately after freezing / thawing the semen and heat resistance during the slow test (TTR). After thawing and end of TTR were found average values of 51%, 58%, 52% and 46% and 31%, 47%, 45% and 44% for treatments D1, D2, D3 and D4 respectively. The addition of the polp fruit of the lyophilized noni in concentrations used, didn’t change the membrane of sperm, recommending studies with a larger number of repetitions to determine its viability.