Croton floribundus Spreng. hydroalcoholic extract efficiency in controlling from bacteria that cause bovine mastitis

Abstract

The medication resistance remains an important world problem. The search for natural therapy is frequently noticed due to the possibility to minimize the use of synthetic chemical medication, as well as the lower occurrence of adverse effects. Capixingui (Croton floribundus) is a tropical tree species considered as a medicinal plant. In its composition, kaurenoic acid stands out, due to it's antimicrobial, cytotoxic and anti-inflammatory action. Thus, the objective was to evaluate the antimicrobial activity of Croton floribundus hydroalcoholic extract at 10% (EHAC10%) on innocuous Staphylococcus aureus ATCC 25923, Staphylococcus saprophyticus ATCC 15305, Escherichia coli ATCC 11229, Klebsiella pneumoniae ATCC 700603 and Pseudomona aeruginosa ATCC 27853. The antimicrobial activity was evaluated using disc diffusion and minimum inhibitory concentration. Complementarily, antioxidant activity was determined, as well as flavonoids and phenols total levels. The EHAC10% inhibited Staphylococcus aureus, Staphylococcus saprophyticus, and Pseudomona aeruginosa growth. Regarding antioxidant activity, phenols and flavonoid total contents, it was possible to verify the dose-dependent pattern, presenting maximum values at 1.000 µg mL^-1. The antioxidant activity was recorded from 250 µg mL^-1, with a maximum value corresponding to 70.56%. The highest levels of total phenols and flavonoids corresponded to 280 mg and 44.41 mg, respectively. Thus, the antibacterial and antioxidant activities of EHAC 10% may represent an important therapeutic potential for the control of bovine mastitis, especially for organic production systems.