In vitro evaluation of the remineralizing effect of two different bioactive principles on bovine tooth enamel using X-Ray fluorescence

F. S. Calazans, R. S. Santos, M. S. Miranda, J. T. de Assis, M. J. dos Anjos


Dental erosion, in general, can be interpreted as a situation of a chronic loss of dental hard tissue that is chemically etched away from the tooth surface by acid and/or chelation without bacterial involvement. Thus, if the erosion process takes place for a period of time, a cavity can be formed (caries lesion). A procedure for inhibiting the formation of erosion and enhance the remineralization process is to use chemical agents. However, the aim of this in vitro study was to evaluate the effects of remineralization on bovine enamel using the of X-ray fluorescence technique (XRF). We obtained 15 specimens (CP) whose were randomly divided into three groups with different treatments of the remineralization: Group 1 - Saliva, Group 2 - Fluoride and Group 3 – CG Tooth Mousse. All measurements of X-ray fluorescence were obtained using commercial equipment (ArtaxTM 200). The evaluated chemicals elements from the XRF spectra were P, Ca e Sr. The remineralization process was first evaluated using the Bonferroni test (=0.05) for comparison of parametric average loss of P, Ca and Sr in each stage of treatment in the groups. Besides that, it was also used the t Student test (=0.05) for comparison between means of different treatment groups. The results show that GC Tooth Mousse group was not effective in remineralization of bovine enamel subjected to erosive challenge in this study. Nevertheles, its performance was similar to saliva, and therefore inferior to fluoride.


X-Ray fluorescence, tooth erosion, tooth remineralization

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